Assoc. Prof. Ruchanee Ampornaramveth

รศ.ทพญ.ดร.รัชนี อัมพรอร่ามเวทย์

D.D.S., Ph.D.

Academic staff: Department of Microbiology

Department

Associate Professor Ruchanee Salingcarnboriboon Ampornaramveth, based at the Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, is a prominent researcher in oral microbiology, infection control, and bone tissue engineering. Her work bridges clinical dentistry and biomedical innovation, with a focus on improving dental care and regenerative therapies. Research Focus Areas • Infection Control in Dentistry: Developing strategies to mitigate microbial contamination in dental environments. • Dental Unit Waterline (DUWL) Decontamination: Investigating methods to prevent biofilm formation and microbial proliferation in dental equipment. • Bone Biology & Tissue Engineering: Creating biomaterials and scaffolds to promote bone regeneration and repair. Key Research Contributions 1. Dental Unit Waterline Decontamination • Iodine-Based Disinfection: Demonstrated that continuous iodine release devices effectively reduce bacterial contamination in DUWLs, maintaining levels below CDC-recommended thresholds. • Plasma Sterilization: Showed that plasma treatment significantly decreases bacterial presence in DUWLs, offering a chemical-free sterilization method. • D-Amino Acids Application: Explored the use of D-amino acids as agents to disperse biofilms in DUWLs, providing an alternative approach to traditional chemical disinfectants. 2. Dental Bioaerosol Analysis • Metataxonomic Studies: Analyzed the composition of dental bioaerosols, identifying saliva and DUWLs as primary bacterial sources. Found that while iodine and plasma treatments control DUWL contamination, they do not significantly alter bioaerosol bacterial composition. 3. Bone Tissue Engineering • Chitosan-Based Scaffolds: Developed chitosan/dicarboxylic acid scaffolds that, with or without human periodontal ligament cells, promote in vivo bone regeneration in animal models. • Epigenetic Modulation: Introduced chitosan-based scaffolds incorporating histone deacetylase inhibitors, enhancing osteogenic differentiation and bone formation. • Scaffold Stability: Investigated the effects of storage conditions on the bioactivity of chitosan-derived scaffolds, providing guidelines for their preservation and use.

Research Profile
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Research of interests

  • Bone biology
  • Tissue engineering
  • Infection control in dentistry
  • Dental unit waterlines decontamination

Publications (5 years)

  1. Ampornaramveth RS, Akeatichod N, Lertnukkhid J, Songsang N.Application of D-amino acids as biofilm dispersing agent in dental unit waterlines. International Journal of Dentistry 2018, Article ID 9413925, 7 pages
  2. Sasanakul P, Ampornaramveth RS, Chivatxaranukul P. Influence of Adjuncts to Irrigation in the Disinfection of Large Root Canals. J Endod. 2019, 45(3):332-337
  3. Sarocha Noopan, Phattranit Unchui, Supitcha Techotinnakorn, and Ruchanee Salingcarnboriboon Ampornaramveth. Plasma Sterilization Effectively Reduces Bacterial Contamination in Dental Unit Waterlines. International Journal of Dentistry. Jul 30, 2019: Article ID 5720204
  4. Sukpaita T, Chirachanchai S, Suwattanachai P, Everts V, Pimkhaokham A, Ampornaramveth RS. In Vivo Bone Regeneration Induced by a Scaffold of Chitosan/Dicarboxylic Acid Seeded with Human Periodontal Ligament Cells.  Int J Mol Sci. 2019 Oct 1;20(19):4883. doi: 10.3390/ijms20194883.
  5. Chaitrakoonthong T, Ampornaramveth R, Kamolratanakul P. Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior. Int J Dent. 2020 Mar 21;2020:4706418. doi: 10.1155/2020/4706418. eCollection 2020.
  6. Kanisa Chantarothorn, Nattakarn Narongchai, Apisara Trairattanapa, Chompoonuch Wongwiriya, Ruchanee S Ampornaramveth. Inclusion of Cotton Fabric in a Package of Metal Instruments Retained Intrapackage Humidity after Steam Sterilization. J Contemp Dent 2019;9(2):64–67.
  7. Yingcharoenthana S, Ampornaramveth R, Subbalekha K, Sinpitaksakul P, Kamolratanakul P. A split-mouth randomized clinical trial to evaluate the effect of local and systemic administration of vitamin C on extraction wound healing. J Oral Sci. 2021 Mar 31;63(2):198-200.
  8. Pittayapat P, Ampornaramveth R, Jirachoksopon C, Suvarnbriksha K, Kattapong S, Pethprasert T, Kungsadalpipob K, Chantarangsu S, Thanyasrisung P, Koottathape N, Tamsailom S, Linsuwanont P, Kasevayuth K, Sakoolnamarka R, Osathanon T, Jansisyanont P. Procedures Used in Managing SARS-CoV-2 Infected Dental Personnel or Patients: A Case Study From a Thai Dental Hospital. Front Oral Health. 2021 Oct 25;2:750394. doi: 10.3389/froh.2021.750394. eCollection 2021.
  9. Indrani Sulistyowati, Teerawat Sukpaita, Chalida Nakalekha Limjeerajarus and Ruchanee Salingcarnboriboon Ampornaramveth. Hydroxamate-Based Histone Deacetylase Inhibitors as Potential Mediators to Induce Dentine Regeneration by Human Dental Pulp Cell. Front. Dent. Med, 28 October 2021 | https://doi.org/10.3389/fdmed.2021.765462
  10. Amnuaiphanit P, Thumbuntu T, Gaewkhiew P, Ampornaramveth RS. Paradigm shift in infection control practices in dental clinics in response to COVID-19 among dental professionals in Thailand. Front Oral Health. 2022 Sep 21;3:979600. doi: 10.3389/froh.2022.979600. eCollection 2022.
  11. Sukpaita T, Chirachanchai S, Chanamuangkon T, Nampuksa K, Monmaturapoj N, Sumrejkanchanakij P, Pimkhaokham A, Ampornaramveth RS. Novel Epigenetic Modulation Chitosan-Based Scaffold as a Promising Bone Regenerative Material. Cells. 2022 Oct 13;11(20):3217. doi: 10.3390/cells11203217.
  12. Sukpaita T, Chirachanchai S, Pimkhaokham A, Ampornaramveth RS. Effect of Storage Time and Temperature on the Bioactivity of a Chitosan-Derived Epigenetic Modulation Scaffold. Mar Drugs. 2023 Mar 12;21(3):175. doi: 10.3390/md21030175.
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